Brooke X. C. Kwai, Annabelle J. Collins, Martin J. Middleditch, Jonathan Sperry, Ghader Bashiri and Ivanhoe K. H. Leung
RSC Med. Chem., 2020
Itaconate is a mammalian antimicrobial metabolite that inhibits the isocitrate lyases (ICLs) of Mycobacterium tuberculosis. Herein, we report that ICLs form a covalent adduct with itaconate through their catalytic cysteine residue. These results reveal atomic details of itaconate inhibition and provide insights into the catalytic mechanism of ICLs.