Saturday, August 11, 2018

A green BODIPY‐based, super‐fluorogenic, protein‐specific labelling agent

Yingche Chen, Kelvin Tsao, Sydney L. Acton, Jeffrey W. Keillor

Angewandte Chemie, 2018
doi: 10.1002/ange.201805482

We report the development of YC23, a novel green BODIPY‐based dimaleimide derivative that undergoes a Fluorogenic Addition Reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells.

Covalent Inhibitors of Monoacylglycerol Lipase Induce Conformational Changes and Proteasomal Degradation

Jordan A. Pham, Thanawat Thaingtamtanha, William McLeish, David Lefebvre, Spencer M. Uguccioni, Roxana Filip, Francesco Gentile, and John Pa...